Vector Sapi

Vector Sapi. SapIlinearized pDAUGHTER vector pDAUGHTER 441SR (inducible T5 promoter strong RBS Kan resistance pUC bacterial origin) Methods 1 Reactions for the exchange of ORF from pMOTHER vectors to pDAUGHTER vector were set up in a single 15ml tube as described above Reactions were incubated for 5 10 20 40 or 60 minutes at room temperature.

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SapI NEB

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Karapan Sapi Vector – Sedang

1 SapI digestion creates a 3nt overhang (GCA) for ligation with the SapIdigested pTXB1 vector (containing a TGC overhang) resulting in an inframe fusion to the Nterminus of an intein The SapI site can be used to add one or more extra amino acid residue(s) to the target protein by including an appropriate sequence (eg add ACC in the reverse primer corresponding to a GGT codon for a.

Construction of the Fusion Plasmid (E6901) NEB

SapI We are excited to announce that we are almost finished with switching all reaction buffers to be BSAfree NEB began switching our BSAcontaining reaction buffers in April 2021 to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymesfor restriction enzymes and some DNA modifying enzymes.